- Jacksonville, North Carolina, is a city in Onslow County, North Carolina, United States. As of 2008, the estimated population stood at 81,863. Jacksonville is the principal city of and is included in the Jacksonville, North Carolina metropolitan area.
- A city in central Arkansas, northeast of Little Rock; pop. 29,916
- A city in southeastern North Carolina, a service town for nearby Camp Lejeune and other military facilities; pop. 66,715
- Florida's largest city; a port and important commercial center in northeastern Florida
- An industrial city and port in northeastern Florida; pop. 735,617
- Jacksonville Amtrak station is a passenger train station in Jacksonville, Florida, United States. It is served by Amtrak's Silver Meteor, Silver Star, and Thruway Motorcoach to Lakeland.